The leader of Hamas Ismail Haniya on Thursday said the US president's decision to consider Jerusalem as the capital of Israel as a "war declaration against Palestinians".
In a speech from Gaza city a day after Mr Trump's announcement, Mr Haniya said the US had "killed" the peace efforts in the region.
"This decision has killed the peace process, has killed the Oslo [accord], has killed the settlement process," he added.
"The US decision is an aggression, a declaration of war on us, on the best Muslim and Christian shrines in the heart of Palestine, Jerusalem."
"We should work on launching an intifada in the face of the Zionist enemy," Mr Haniya said.
His calls for a new intifada or uprising came amid protests across the West Bank and Gaza as Palestinians demonstrated against the US decision.
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